FIG. 6.

Binding of AFP1 to chitin, chitosan, and fungal cell walls. Fluorescein-labeled AFP1 was incubated overnight with crab shell chitin (A), crab shell chitosan (B), P. variotii cell wall (C), β-1,3-glucan (curdlan; D), Avicel (E), and xylan (F). After three washes with 100 mM Tris-HCl (pH 7.6)–150 mM NaCl, samples were analyzed by phase-contrast (left) and fluorescence (right) microscopy.