Fig. 5. Michaelis–Menten trial with varying glucose concentration, while keeping MES buffer, GOx and nanosheet concentrations fixed. (a) Absorbance at 384 nm vs. time for various glucose concentrations. (b) Initial reaction rate vs. glucose concentration for data shown in (a), along with a least square fit to eqn (1). The fitting yield 75 μL of 7.5 mg mL⁻¹ GOx is injected into a 4 mL solution in a UV-Vis cuvette at the 30 s mark and stirred.