Fig. 2. Effect of miR-24 on apoptosis in vitro: (A) confocal images of cardiomyocytes transfected with naked miR-24, PEI-miR-24 and F-silica-miR-24. The nucleus was stained blue with DAPI, and miR-24 was labeled with green fluorescent FAM (bars = 20 μm). (B) A cell viability assay estimated the H₂O₂-induced cytotoxicity. (C) The expression of miR-24 was detected by real-time PCR. (D and E) The expression of Bim protein was detected by western blotting (^#p < 0.05 compared with the control group; ^$p < 0.05 compared with the H₂O₂ group).