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Cell culture and cell viability. (a) Experimental procedure of cell culture, perfusion, circulation, and treatments in the GLA-MPS. (b) Evaluation of cell viability of Caco-2 cells (2 μl min−1 of the perfusion flow; 61 nl min−1 of circulation flow) and HepG2 cells (without perfusion flow; 61 nl min−1 of circulation flow) in the GLA-MPS.
