TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant genotype or characteristic | Source or reference |
|---|---|---|
| Strains | ||
| ECL1 | HfrC fuc+ crp+ | 7 |
| ECL56 | HfrC fucAO(Con) IS5-fucPIK crp201 | 24 |
| CSH100 | F′ lac proA+B+ (lacIqlacPL8)/araD (gpt-lac)5 | Cold Spring Harbor Laboratory |
| CSH100 fucAO(Con) IS5-fucPIK | fucAO(Con) IS5-fucPIK from ECL56 | This study |
| FW102 | F−/araD (gpt-lac)5 Strr | 21 |
| JCB43Δcrp39 | F−/Δ(gpt-proAB-arg-lac)XIII Δcrp39 | 13 |
| JCB43Δcrp39[F′ pfuc-lacZ] | fucPIK promoter-lacZ fusion on F′ | This study |
| JCB43Δcrp39[F′ IS5 pfuc-lacZ] | IS5-containing fucPIK promoter-lacZ fusion on F′ | This study |
| JCB43Δcrp39[F′ crp1 pfuc-lacZ] | CRP site 1 in cis to fucPIK promoter inactivated | This study |
| JCB43Δcrp39[F′ IS5-crp1 pfuc-lacZ] | CRP site 1 in cis to IS5-containing promoter inactivated | This study |
| ECL733 | F−/Δfuc ΔlacU169 | 25 |
| ECL733Δcrp39 | Δcrp39 from JCB43Δcrp39 | This study |
| ECL733Δcrp39[F′ pfuc-lacZ] | fucPIK promoter-lacZ fusion on F′ | This study |
| ECL733Δcrp39[F′ IS5 pfuc-lacZ] | IS5-containing fucPIK promoter-lacZ fusion on F′ | This study |
| ECL733Δcrp39[F′ crp1 pfuc-lacZ] | CRP site 1 in cis to fucPIK promoter inactivated | This study |
| ECL733Δcrp39[F′ IS5-crp1 pfuc-lacZ] | CRP site 1 in cis to IS5-containing promoter inactivated | This study |
| Plasmids | ||
| pHA7Ea | Directs expression of crp+ | 12 |
| pHA7-K52Na | Directs expression of crpK52N | 12a;1. |
| pHA7-H159La | Directs expression of crpH159L | 12a |
| pHA7-K52N,H159La | Directs expression of crpK52N,H159L | 12a |
| pFW11-nullb | Contains promoterless lacZ fragment | 21 |
a
Provides resistance to carbenicillin.
b
Provides resistance to chloramphenicol.