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. 2022 Aug 27;6:38. doi: 10.1038/s41538-022-00156-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a AB-PAS staining of ileum and colon, Scale bar: 100 μm; b immunohistochemistry of occludin and ZO-1 protein in ileum, Scale bar: 200 μm; c the α-diversity index (chao1) of gut microbiota; d the β-diversity analysis (PCoA) of gut microbiota; e relative abundance of biomarker genra in family level and genus level. Data are expressed as mean ± standard deviation. One-way ANOVA was used to analyze statistical differences; compared with control group, ^#P < 0.05, ^##P < 0.01; compared with HFD group, ^*P < 0.05, ^**P < 0.01.