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. 2022 Aug 27;12:14636. doi: 10.1038/s41598-022-18675-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The histomorphological and immunohistochemical features of the bone-like tissues. (a) HE staining showed osteoblast-like cells surrounded by a cohesive osteoid-like matrix and osteocyte-like cells embedded into lacunae-like structures (black arrowheads) in the 3:1, 2:1, and 1:1 co-cultures. (b,c) Osteoblast-like cells on the osteoid-like matrix surface were positive for ALP (blue arrowheads) and Col1 (orange arrowheads). (d) Osteocyte-like cells in the osteoid-like matrix were positive for DMP1 (green arrowheads). (e,f) Fluorescence microscopy showed that osteocyte-like cells embedded in the osteoid-like matrix and osteoblast-like cells on the surface were GFP positive (red arrowheads). DAPI, 4′,6-diamidino-2-phenylindole.