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. 2022 Aug 27;13(8):740. doi: 10.1038/s41419-022-05175-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A The effect of shRNAs was validated in SHG-44, A172, and U251 glioma cell lines. B The overexpression efficiency of circKIF4A was examined by qPCR analysis. C, D Knockdown and overexpression of circKIF4A significantly decreased and increased the cell proliferation rate evaluated by CCK-8 assays in SHG-44, A172, and U251 glioma cell lines. E, F Inhibition of circKIF4A attenuated the colony forming ability of three glioma cell lines assessed by colony-formation experiments. G, J The cell proliferation rate was evaluated by an EdU assay. H, K Silencing of circKIF4A decreased the wound closure percentage due to the decreased cell migration ability assessed by a wound-healing assay in the A172 glioma cell line. I, L Overexpression of circKIF4A increased the relative migration cells because of the increased cell migration ability evaluated by transwell assays in the SHG-44 glioma cell line. M The glucose uptake rate of three glioma cell lines was reduced after transfection with shRNA. N The lactate production rate of three glioma cell lines was increased after overexpressing circKIF4A. O, P Inhibition of circKIF4A remarkably enhanced the sensitivity of glioma to temozolomide treatment in SHG-44 and U251 glioma cell lines. *p < 0.05. **p < 0.01. The experiment was repeated three times independently.