Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Aug 27;13(8):739. doi: 10.1038/s41419-022-05191-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Crown 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — A Left panel: fold changes (relative to normoxia) in cellular necrosis (% Annexin-V⁺ PI⁺ cells) for PTEC cultured under normoxic and hypoxic conditions. Bar graphs represent mean ± SEM. Symbols represent individual donor PTEC; n = 4. *P < 0.05, paired t test. Right panel: representative donor Annexin-V/PI dot plots of PTEC cultured under normoxic and hypoxic conditions. The percentage of Annexin-V⁺ PI⁺ necrotic cells for each dot plot are presented, with fold change (FC) value relative to normoxic PTEC also shown. B Left panel: fold changes (relative to normoxia) in GPX4 protein levels (as a ratio of loading control β-tubulin) for PTEC cultured under normoxic and hypoxic conditions. Bar graphs represent mean ± SEM. Symbols represent individual donor PTEC; n = 4. **P < 0.01, paired t test. Right panel: GPX4 western blot for PTEC cultured under normoxic and hypoxic conditions (15 µg total protein per lane). Representative images from one of four donor PTEC are presented; full and uncropped western blot available as Supplementary Material. C Left panel: fold changes (relative to normoxia) in 4-HNE levels (measured as mean corrected total cellular fluorescence (CTCF) of >60 cells per condition) for PTEC cultured under normoxic and hypoxic conditions. Bar graphs represent mean ± SEM. Symbols represent individual donor PTEC; n = 6. *P < 0.05, paired t test. Right panel: immunofluorescent labelling of representative PTEC cultured under normoxic and hypoxic conditions and stained for 4-HNE (green), β-tubulin (red) and DAPI (blue). 4-HNE positivity is highlighted with white arrows. Scale bars represent 20 µm. D Left panel: representative donor bar graph of cellular necrosis for PTEC cultured under normoxic (N) and hypoxic (H) conditions in the absence (-Fer-1; DMSO vehicle control) or presence of ferrostatin-1 (+Fer-1). Right panel: fold changes (relative to hypoxia/-Fer-1) in cellular necrosis (% Annexin-V⁺ PI⁺ cells) for hypoxic PTEC cultured in the absence or presence of ferrostatin-1. Bar graphs represent mean ± SEM. Symbols represent individual donor PTEC; n = 7. *P < 0.05, paired t test.