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. 2022 Aug 27;13:5062. doi: 10.1038/s41467-022-32477-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Dot plot summarizing albuminuria (urinary albumin-creatinine ratio, µg albumin/mg creatinine; UACR) in non-diabetic (C, n = 9) or diabetic (DM) wild-type (Wt, n = 8), TM^Pro/Pro (n = 7) and TM^Pro/Pro x p21^−/− (n = 8) mice. b Exemplary histological images of periodic acid Schiff stains (PAS) showing tubuli (top) or glomeruli (2^nd row), interstitial fibrosis (Masson’s trichrome stain, MTS), SA-β-gal. stain (senescence associated β-galactosidase, blue; eosin counterstain), and γ-H2A.X immunohistochemistry (histone H2A family X, red; DAPI nuclear counterstain, insets: larger magnification) in experimental groups (as described in a); all scale bars represent 20 μm. c, d Dot plots summarizing tubular fibrotic area (c) and fractional mesangial area (FMA, d), the latter reflecting glomerulosclerosis in experimental groups (as described in a). e Exemplary immunoblot (bottom; loading control: GAPDH) and dot plot summarizing results (top) of renal KIM-1 expression in experimental groups (as described in a). f, g Dot plots summarizing percentage of SA-β-gal. positive area (f) and percentage of γ-H2A.X positive cells (g) in experimental groups (as described in a). h, i Exemplary histological images of glomerular nephrin expression (green, DAPI nuclear counterstain, blue, h), and dot plot summarizing nephrin staining intensity fold change (i) in experimental groups (as described in a). Dot plots reflecting mean ± SEM of six mice per group; one-way ANOVA with Sidak’s multiple comparison test. Source data are provided as a Source Data file-Fig-5.