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. 2022 Aug 27;8:375. doi: 10.1038/s41420-022-01166-3

Fig. 4. NETs impair the cell viability via inducing autophagosome formation but blocking autophagic flux.

Fig. 4

A The cell viability of alveolar epithelial cells with different treatments. B Representative electron microscopic images of autophagic vesicles (black arrow) in alveolar epithelial cells with different treatments. C Representative images of immunofluorescence staining of LC3B in alveolar epithelial cells with different treatments. Scale bar: 20 µm. D Western blot images of autophagy signaling and cell death markers expression in alveolar epithelial cells. E Representative fluorescent images of alveolar epithelial cells transfected with mCherry-EGFP-LC3B and treated with EBSS or NETs. Scale bar: 10 µm. Each bar shows means ± SEM. The comparison between two groups was conducted by unpaired t-test. *p < 0.05, **p < 0.01 versus the control group.