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. 2022 Aug 27;8:375. doi: 10.1038/s41420-022-01166-3

Fig. 5. Activation of autophagic flux restores cell viability.

Fig. 5

A The cell viability of alveolar epithelial cells with different treatments. B Western blot images of autophagy signaling and cell death markers expression in alveolar epithelial cells with different treatments. C The mRNA level of Sirt1 in alveolar epithelial cells. D Western blot images of SIRT1 expression in alveolar epithelial cells. E Representative fluorescent images of alveolar epithelial cells transfected with mCherry-EGFP-LC3B and treated with NETs or NETs+SRT1720. Scale bar: 10 µm. Each bar shows means ± SEM. The data comparison between two groups was analyzed based on unpaired t-test. *p < 0.05, **p < 0.01 versus the control group. #p < 0.05, ##p < 0.01 versus the NETs group.