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. 2022 Aug 27;8:375. doi: 10.1038/s41420-022-01166-3

Fig. 7. METTL3 knockdown activates autophagic flux and restores the mRNA stability of Sirt1.

Fig. 7

The alveolar epithelial cells were transfected with si-Control or si-METTL3 and treated with NETs. A The mRNA levels of Sirt1 expression in alveolar epithelial cells. B Western blot images of SIRT1 and autophagy signaling expression in alveolar epithelial cells. C Representative fluorescent images of alveolar epithelial cells transfected with mCherry-EGFP-LC3B and treated with NETs. Scale bar: 10 µm. D Representative images of immunofluorescence co-staining of METTL3 and SIRT1 in alveolar epithelial cells. Scale bar: 30 µm. E The methylated mRNA levels of Sirt1 in alveolar epithelial cells. F The stability of Sirt1 mRNA in alveolar epithelial cells. Each bar shows means ± SEM. The comparison between two groups was performed using unpaired t-test. **p < 0.01 versus the si-Control group. #p < 0.05, ##p < 0.01 versus the si-Control-NETs group.