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TABLE 1.

Aggregation of M. tuberculosis H37Ra in the presence of purified native and recombinant HBHAa

HBHA protein Amt of HBHA that induces maximum aggregation (μg/ml)
Native HBHA 0.5
h-HBHA 2.5
N-h-HBHA 12.5
C-h-HBHA >50
h-MPT64 >50
a

Native HBHA and His-tagged recombinant HBHA proteins were purified as described in Materials and Methods. The His-tagged recombinant mycobacterial protein h-MPT64 (28) was purified in a similar fashion and used as a control.