TABLE 1.
HBHA protein | Amt of HBHA that induces maximum aggregation (μg/ml) |
---|---|
Native HBHA | 0.5 |
h-HBHA | 2.5 |
N-h-HBHA | 12.5 |
C-h-HBHA | >50 |
h-MPT64 | >50 |
Native HBHA and His-tagged recombinant HBHA proteins were purified as described in Materials and Methods. The His-tagged recombinant mycobacterial protein h-MPT64 (28) was purified in a similar fashion and used as a control.