Table 2. In Vitro Antioxidant Activity of the Investigated Lemon Balm Extracts and Positive Control Substances a, b, c.
Samples | Assay | ||||
---|---|---|---|---|---|
DPPH IC50 | OH IC50 | NO IC50 | LP IC50 | FRAP (mg AAE/g d.e.) | |
E1 | 9.95 ± 0.39 A,B | 39.99 ± 3.14 A,B | 36.67 ± 3.75 | 50.54 ± 4.16 A,B | 341.71 ± 42.69 B |
E2 | 4.76 ± 0.48 A | 55.97 ± 4.18 A,C | 35.34 ± 3.11 | 81.10 ± 6.47 A,C | 329.06 ± 23.75 C |
E3 | 4.91 ± 0.49 B | 61.34 ± 4.87 B,C | 32.87 ± 3.01 | 58.53 ± 5.15 B,C | 294.39 ± 11.67 B,C |
AA | / | 2.03 ± 0.39* | / | / | / |
PG | 0.67 ± 0.02* | 8.94 ± 0.45* | 9.12 ± 0.27* | / | / |
BHT | / | 0.04 ± 0.00* | / | 7.08 ± 0.23* | / |
Abbreviations: OH, hydroxyl radicals; NO, nitroso radicals; LP, lipid peroxidation inhibition potential; FRAP, ferric reduction antioxidant potential; AAE, ascorbic acid equivalents; PG, propyl gallate; BHT, tert-butylated hydroxytoluene; SD, standard deviation.
a Values are expressed as mean ± SD µg/mL unless otherwise indicated.
bDPPH, the ability of the examined lemon balm extracts to neutralize 2,2-diphenyl-1-picrylhydrazyl radical; E1: Ethanolic (70%) macerate; E2: Aqueous extract obtained under solvent reflux; E3: Ethanolic (70%) extract obtained under solvent reflux.
cA, B, C sharing the same letter denotes groups' statistically significant differences; * Denotes statistically significant differences between standards and all three extracts (E1, E2, and E3).