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. 2022 May 5;21(1):e126561. doi: 10.5812/ijpr-126561

Table 2. In Vitro Antioxidant Activity of the Investigated Lemon Balm Extracts and Positive Control Substances a, b, c.

Samples Assay
DPPH IC50 OH IC50 NO IC50 LP IC50 FRAP (mg AAE/g d.e.)
E1 9.95 ± 0.39 A,B 39.99 ± 3.14 A,B 36.67 ± 3.75 50.54 ± 4.16 A,B 341.71 ± 42.69 B
E2 4.76 ± 0.48 A 55.97 ± 4.18 A,C 35.34 ± 3.11 81.10 ± 6.47 A,C 329.06 ± 23.75 C
E3 4.91 ± 0.49 B 61.34 ± 4.87 B,C 32.87 ± 3.01 58.53 ± 5.15 B,C 294.39 ± 11.67 B,C
AA / 2.03 ± 0.39* / / /
PG 0.67 ± 0.02* 8.94 ± 0.45* 9.12 ± 0.27* / /
BHT / 0.04 ± 0.00* / 7.08 ± 0.23* /

Abbreviations: OH, hydroxyl radicals; NO, nitroso radicals; LP, lipid peroxidation inhibition potential; FRAP, ferric reduction antioxidant potential; AAE, ascorbic acid equivalents; PG, propyl gallate; BHT, tert-butylated hydroxytoluene; SD, standard deviation.

a Values are expressed as mean ± SD µg/mL unless otherwise indicated.

bDPPH, the ability of the examined lemon balm extracts to neutralize 2,2-diphenyl-1-picrylhydrazyl radical; E1: Ethanolic (70%) macerate; E2: Aqueous extract obtained under solvent reflux; E3: Ethanolic (70%) extract obtained under solvent reflux.

cA, B, C sharing the same letter denotes groups' statistically significant differences; * Denotes statistically significant differences between standards and all three extracts (E1, E2, and E3).