Figure 1. Generation of Rnf144a knockout-first and exons-deletion mutant mice.

(A-D) Schematic diagrams show the allele of WT Rnf144a (A), the targeted Knockout-first Rnf144a^βgeo (B), the conditional Rnf144a^flox (C), and the knockout Rnf144a^ko (D).

(E) PCR genotyping of genomic DNA confirms the deletion of a fragment of the Rnf144a gene in Rnf144a^ko alleles.

(F) Western blot analysis confirms the absence of RNF144A protein in the bladder tissues of RNF144A-deficient mice.

(G-H) No significant change in body size (G) and body weight (H) between Rnf144a WT and KO mice.