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. 2022 Aug 19;3(3):101642. doi: 10.1016/j.xpro.2022.101642

Size exclusion chromatography co-elution buffer

Reagent Final concentration Amount
Tris-HCl, pH7.5 (1 M) 20 mM 20 mL
NaCl (5 M) 100 mM 100 mL
TCEP (0.5 M) 1 mM 2 mL
ddH2O N/A 878 mL
Total N/A 1,000 mL

Store at 4°C within 14 days.