PCR cycling conditions
| Steps | Temperature | Time | Cycles |
|---|---|---|---|
| Initial Denaturation | 95°C | 2 min | 1 |
| Denaturation | 95°C | 20 s | 30 cycles |
| Annealing | 56°C | 10 s | |
| Extension | 70°C | 15 s | |
| Hold | 4°C | forever | |
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