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. 2022 Aug 19;3(3):101642. doi: 10.1016/j.xpro.2022.101642

Lysis buffer A

Reagent Final concentration Amount
Tris-HCl, pH7.5 (1 M) 50 mM 50 mL
NaCl (5 M) 500 mM 100 mL
DTT (1 M) 0.2 mM 0.2 mL
ddH2O N/A 849.8 mL
Total N/A 1,000 mL

Store at 4°C within 14 days.