Reagent | Final concentration | Amount |
---|---|---|
Tris-HCl, pH7.5 (1 M) | 50 mM | 50 mL |
NaCl (5 M) | 500 mM | 100 mL |
DTT (1 M) | 0.2 mM | 0.2 mL |
ddH2O | N/A | 849.8 mL |
Total | N/A | 1,000 mL |
Store at 4°C within 14 days.
Reagent | Final concentration | Amount |
---|---|---|
Tris-HCl, pH7.5 (1 M) | 50 mM | 50 mL |
NaCl (5 M) | 500 mM | 100 mL |
DTT (1 M) | 0.2 mM | 0.2 mL |
ddH2O | N/A | 849.8 mL |
Total | N/A | 1,000 mL |
Store at 4°C within 14 days.