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. 2022 Aug 13;56:102431. doi: 10.1016/j.redox.2022.102431

Fig. 7.

Fig. 7

The effect of LIX1 silencing on mitochondrial morphology in GIST-T1 cells is reversed by linoleic acid.

(A) Representative Western blot showing TAZ and αSMA levels in GIST-T1-Scrambled (+ETOH vehicle), GIST-T1-ShLIX1#2 (+EtOH vehicle) and -ShLIX1#2 (+50 μM of linoleic acid) cells. Equal loading was verified by GAPDH expression. (B) Quantification of the Western blot bands to determine TAZ and αSMA expression levels relative to GAPDH in the different experimental conditions. Normalized expression levels were converted into fold change. Values are the mean ± SEM of n = 3 samples for each condition. *P < 0.05 (two-tailed Mann–Whitney test). (C) MitoTracker staining of GIST-T1-Scrambled (+EtOH vehicle), GIST-T1-ShLIX1#2 (+EtOH vehicle), and -ShLIX1#2 cells (+50 μM of linoleic acid). Nuclei were visualized with Hoechst. Scale bars, 10 μm. (D) Quantification of the MitoTracker data with the Mito-Morphology Macro in Image J. Values are the mean ± SEM of GIST-T1-Scrambled (+DMSO vehicle) (n=25), GIST-T1-ShLIX1#2 (+DMSO vehicle) (n=28), and GIST-T1-ShLIX1#2 cells (+50 μM of linoleic acid) (n=28); ****P < 0.0001 (two-tailed Mann–Whitney test). (E) Ultrastructure of mitochondria in GIST-T1-Scrambled (+EtOH vehicle), GIST-T1-ShLIX1#2 (+EtOH vehicle), and -ShLIX1#2 (+50 μM of linoleic acid) cells. Lower panels: magnification of the areas in the black boxes in the upper panels.