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. Author manuscript; available in PMC: 2023 Jun 1.
Published in final edited form as: Shock. 2022 Jun 1;57(6):218–227. doi: 10.1097/SHK.0000000000001928

Fig. 3. DNMT mRNA expression in monocytes exposed to exosomes isolated from LPS stimulated monocytes (monocyteLPS-exosome).

Fig. 3.

CD14+ monocytes were isolated from human PBMC. A, DNMT1 levels were measured using RT-PCR in naïve recipient human monocytes after 12 h incubation with exosomes derived from human monocytes treated with PBS (control, n=5) or with LPS (6 ng/mL, n=7). Recipient cells were also treated with pharmacologic uptake inhibitors wortmannin (n=4) or latrunculin (n=10). B, DNMT3a levels were measured under the same conditions. The data shown are mean±SD with quartiles. One-tailed t test analysis was performed for comparisons. *P<0.01, **P=0.03.