Figure 2. Retrograde tracing from CGRP^SPFp and CGRP^PBel neurons.

(A) Schematic of monosynaptic retrograde rabies tracing experiment and example histology image of the CGRP^SPFp (left) and CGRP^PBel (right) starter cells. Scale bars, 200 μm.

(B) Example images of spinal dorsal horn and brain regions that send inputs to CGRP^SPFp (top) and CGRP^PBel (bottom) neurons. Scale bars, 100 μm.

(C) Percentage of total projections from each brain region to CGRP^SPFp (left) and CGRP^PBel (right) neurons. n = 5 (CGRP^SPFp), n = 6 mice (CGRP^PBel).

(D) Diagrams of brain-wide inputs to CGRP^SPFp (top) and CGRP^PBel (bottom) neurons and their relative percentages.

SDH, spinal dorsal horn; CB, cerebellum including interposed nucleus (IP), arbor vitae (arb); SPV, trigeminal spinal nucleus; RN, reticular nucleus including the nucleus tractus solitarius (NTS), intermediate reticular nucleus (IRN), parvicellular reticular nucleus (PARN), gigantocellular reticular nucleus (GRN); VN, vestibular nucleus including medial vestibular nucleus (MV), lateral vestibular nucleus (LAV), spinal vestibular nucleus (SPIV); Pons including the nucleus of the lateral lemniscus, pontine central gray, parabrachial nucleus, pontine reticular nucleus (PRN); MRN, midbrain reticular nucleus; IC, inferior colliculus; SC, superior colliculus; PAG, periaqueductal gray; PRT, pretectal region including anterior pretectal nucleus (APN), medial pretectal area (MPT), nucleus of the posterior commissure (NPC), olivary pretectal nucleus (OP); SNr, substantia nigra; VTA, ventral tegmental area; HY, hypothalamus including lateral hypothalamus (LHA), zona incerta (ZI); Amy, amygdala; CTX, cortex; CP, striatum; GP, globus pallidus; HPF, hippocampus; TH, thalamus; BST, bed nuclei of the stria terminalis; SI, substantia innominata; sptv, spinal tract of the trigeminal nerve; cpd, cerebral peduncle. Data are presented as mean ± SEM. See also Figure S3; Tables S2 and S3.