Figure 3.

Spike-independent attenuation of the Omicron variant in Balb/c mice

(A) Overall study design.

(B) Weight loss profile.

(C) Clinical scores of all three groups of mice overall the period of study.

(D–G) Subgenomic RNA (sgmRNA) and total viral RNA levels in nasal turbinates (D and E) and lungs (F and G).

(H) Induction of ISG15 at 2 DPI in mouse lungs.

(I) Summed pathology scores of all three groups of mice overall the period of study.

(J–M) Representative H&E images of the natural Omicron-infected mice. (K) is a closeup image from (J), and (M) is a closeup from (L).

(N) An RNAscope image of the natural Omicron variant-infected mouse.

(O) A closeup from (N). Blue, nuclei; red, viral RNA.

(P) A representative H&E image of the WA1-Omicron S-infected mouse.

(Q) A closeup from (P).

(R) A representative RNAscope image of the WA1-Omicron S-infected mouse.

(S) A closeup from (R).

(T) A representative H&E image of the WA1-Q493R/N501Y-infected mouse.

(U) A closeup from (T).

(V) A presentative RNAscope image of the WA1-Q493R/N501Y-infected mouse.

(W) A closeup from (V).

Results shown in this figure were derived from one experiment (n = 10–15 for each group). Each solid shape represents one animal in (D)–(H).