REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
SARS-Coronavirus 2, Isolate USA-WA1/2020	BEI Resources	Cat#NR-52281
SARS-Coronavirus 2, Isolate hCoV-19/USA/MD-HP20874/2021	BEI Resources	Cat#NR-56461
SARS-Coronavirus 2, WA1-Omicron-S	This manuscript	N/A
SARS-Coronavirus 2, WA1-Q493R/N501Y	This manuscript	N/A
Endura™ Chemically Competent Cells	Lucigen	Cat#60240-2
TransforMax™ EPI300™ Competent Cells	Lucigen	Cat#C300C105
Critical commercial assays
Lipofectamine-3000 Transfection Reagent	ThermoFisher	Cat#L3000015
Luciferase Assay System	Promega	Cat#E1501
SuperScript IV Reverse Transcriptase	ThermoFisher Scientific	Cat#18090010
Phusion® High-Fidelity DNA Polymerase	New England Biolabs	Cat#M0530L
mMESSAGE mMACHINE™ T7 Transcription Kit	ThermoFisher Scientific	Cat#AM1344
Experimental models: Cell lines
Lenti-X™ 293T Cell Line	Takara	Cat#632180
Vero (C1008) E6	ATCC	Cat#CRL-1586
Oligonucleotides
Primer M13 F (GTAAAACGACGGCCAGT)	Facility for Biotechnology Resources, FDA	N/A
Primer SARS-2S-PRRA F (AGACAAACTC CCGGTCTGTGGCAAGCCAGTC)	Facility for Biotechnology Resources, FDA	N/A
Primer Q493R F (TTGTTACTTTCCTTTAA GATCATATGGTTTCCAACCCACT)	Facility for Biotechnology Resources, FDA	N/A
Primer Q493R R (GAAACCATATGATCTT AAAGGAAAGTAACAATTAA)	Facility for Biotechnology Resources, FDA	N/A
Primer N501Y F (GTTTCCAACCCACTT ATGGTGTTGGTTACCAACCATA)	Facility for Biotechnology Resources, FDA	N/A
Primer N501Y R (ACCAACACCATAA GTGGGTTGGAAACCATATGA)	Facility for Biotechnology Resources, FDA	N/A
Primer BglII R (CAGCATCTGCAAGTGTCACT)	Facility for Biotechnology Resources, FDA	N/A
Recombinant DNA
pcDNA-human-ACE2	GenScript	N/A
pcDNA-mouse-ACE2	GenScript	N/A
pcDNA-ferret-ACE2	GenScript	N/A
pcDNA-Syrian hamster-ACE2	GenScript	N/A
pcDNA-Grivet (African green monkey)-ACE2	GenScript	N/A
pcDNA-horseshoe bat-ACE2	GenScript	N/A
pcDNA-pig-ACE2	GenScript	N/A
pcDNA-bovine-ACE2	GenScript	N/A
pcDNA-Chinese hamster-ACE2	GenScript	N/A
pcDNA- Malayan pangolin -ACE2	GenScript	N/A
pcDNA- SARS-CoV-2 Wuhan Spike	This manuscript	N/A
pcDNA- SARS-CoV-2 Omicron Spike	This manuscript	N/A
pcDNA- SARS-CoV-2 Spike N501Y-Q493K	This manuscript	N/A
pcDNA- SARS-CoV-2 Spike N501Y-Q493R	This manuscript	N/A
pcDNA- SARS-CoV-2 Spike G496K-Q493K	This manuscript	N/A
pcDNA- SARS-CoV-2 Spike N501Y-S494R	This manuscript	N/A
pcDNA- SARS-CoV-2 Spike N501Y-G496K	This manuscript	N/A
pcDNA- SARS-CoV-2 Spike N501F-G496K	This manuscript	N/A
pcDNA- SARS-CoV-2 Spike N501K-Q493K	This manuscript	N/A
pcDNA- SARS-CoV-2 Spike Q493K-Q498Y-P499T	This manuscript	N/A
TMPRSS2	Addgene	Cat#53887
psPAX2	Addgene	Cat#12260
pCMV-CREM	Addgene	Cat#8395
pSV-STOP-luc	Addgene	Cat#8390
RNAscope reagents
RNAscope 2.5 HD RED kit	ACD	Cat#322373
V-SARS-Cov-2-S	ACD	Cat#854841
Mm PPIB probe (positive control)	ACD	Cat#313911
dapB probe (negative control)	ACD	Cat#310043
Software and algorithms
Prism 9.0 software	GraphPad	N/A
SnapGene	GSL Biotech LLC	N/A