Table 1.
Targeted genes with their primer sequences and annealing temperature.
| Gene | Primer Sequences (5′-3′) | Annealing Temperature (°C) |
|---|---|---|
| Interleukin 1 beta (IL-1β) | F: TCATCTTTGAAGAAGAGCCCGT R: TCATCTTTGAAGAAGAGCCCGT |
58 |
| Interleukin 6 (IL-6) | F: GATGGATGCTTCCAAACTGGATA R: TGAATGACTCTGGCTTTGTCTTT |
58 |
| Interleukin 4 (IL-4) | F: CGTGATGTACCTCCGTGCTT R: ATTCACGGTGCAGCTTCTCA |
58 |
| Interleukin 5 (IL-5) | F: TGATACAGCTGTCCACTCACC R: TGCCCACTCTGTACTCATCAC |
58 |
| Glutathione peroxidase 7 (GPX7) | F: AGGAGATCAAGCCCCGTAT R: CGAAGGGAAGAGAAGCATT |
58 |
| Peroxiredoxin 7 (PRDX7) | F: CAAAGCCACGGCTGTTATGC R: TGGGTCCCAATCCTCCTTGT |
58 |
| Thioredoxin reductase 2 (TXNRD2) | F: GCCCAGTACAAGGCTCTCA R: AGGTTAGAGGGGGCAGTCA |
58 |
| Vascular endothelial growth factor (VEGF) | F: TGAATGACTCTGGCTTTGTCTTT R: TGAATGACTCTGGCTTTGTCTTT |
58 |
| Fibroblast growth factor (FGF) | F: AGCAGAAGAGAGAGGAGTTGTG R: TATTTCCGTGACCGGTAAGTGT |
58 |
| Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | F: GGAAAGCTGTGGCGTGATGG R: GTAGGCCATGAGGTCCACCA |
58 |
| F = forward primer, R = reverse primer | ||