FIGURE 1.

(A) Effect of PMA on VEGF intracellular content in HUVEC cells. Cells were exposed to PMA (100 nM) for 48 h. Densitometric analysis of VEGF protein levels. The results are expressed as mean grey levels ratios (mean ± S.D.) of VEGF/α-tubulin immunoreactivities × 1000 measured by Western blotting (upper side: cropped Western blotting images and lower side: densitometric analysis). (B) Effect of PMA on VEGF release in the medium of HUVEC cells. Cells were exposed to PMA (100 nM) for 48 h. VEGF protein levels were measured by ELISA. The results are expressed in pg/mL (mean ± S.D.). *p < 0.05, Student’s t-test for both intracellular and released VEGF, n = 4 independent experiments. CTR, control; PMA, phorbol 12-myristate 13-acetate.