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. 2022 Aug 15;13:951833. doi: 10.3389/fphar.2022.951833

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Copyright © 2022 Fahmideh, Marchesi, Campagnoli, Landini, Caramella, Barbieri, Govoni and Pascale.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Combined effect of high glucose and troxerutin on PKCβII and HuR in HREC. PKCβII and HuR were evaluated in the total homogenates of HREC following exposure for 72 h (A,B) and 7 days (C,D) to high glucose levels (25 mM) with or without troxerutin (1 mM). Upper side: cropped Western blotting images. Lower side: densitometric analysis. The results are expressed as mean grey levels ratios (mean ± S.D.) of PKCβII/α-tubulin and HuR/α-tubulin immunoreactivities × 1000 measured by Western blotting. *p < 0.05, **p < 0.01, Dunnett’s Multiple Comparisons test, n = 4–8 independent experiments. CTR, control; HG, high glucose; Troxo, troxerutin.