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. 2022 Aug 23;31:09636897221077928. doi: 10.1177/09636897221077928

Figure 3.

Figure 3.

NLRP3 is directly targeted by miR-223-3p. (A) Bioinformatics programs including RegRNA, miRTarBase, and TargetScan predicted miR-223-3p was the potential miRNA combining with NLRP3. (B) RT-qPCR examined miR-223-3p expression in normal and hypoxia HCMs. (C) MiR-223-3p expression was detected in hypoxia HCMs transfected with miR-223-3p mimics. (D, E) RT-qPCR and WB examined NLRP3 expression after miR-223-3p upregulation in hypoxia HCMs or after miR-223-3p inhibition in normal HCMs. (F) Ago2-RIP assay indicated the enrichment of NLRP3 and miR-223-3p in Ago2 groups. (G) RNA pull-down experiments showed the enrichment of NLRP3 in Biotin-miR-223-3p WT groups. (H) StarBase predicted the binding site between NLRP3 and miR-223-3p. (I) The affinity between NLRP3 and miR-223-3p was further investigated via luciferase reporter experiments. HCM: human cardiomyocytes; miRNA: microRNA; NLRP3: NLR family pyrin domain containing 3; RT-qPCR: reverse transcription polymerase chain reaction; WT: wild type. **P < 0.01.