FIG. 4.

Localization of FtsZ and its derivatives in JFL101 [ftsZ84(Ts)] cells. JFL101 derivatives containing various plasmids were grown at 42°C for 30 min, induced with 10 μM IPTG for 60 min, and then fixed for staining. Simultaneously, FtsZ was stained by anti-FtsZ and visualized by IFM (A to D), nucleoids were stained with DAPI and visualized by fluorescence microscopy (A′ to D′), and cell morphologies were visualized by phase-contrast microscopy (A" to D"). (A to A") JFL101 containing pWM176; (B to B") JFL101 containing pMK4 (wild-type ftsZ); (C to C") JFL101 containing pWM1202 (ZL372A); (D to D") JFL101 containing pWM932 (ZΔC1.3).