FIG. 6.

Association of FtsA-GFP with FtsZ spirals in JFL101 [ftsZ84(Ts)]. JFL101 cells containing plasmids synthesizing FtsA-GFP and an FtsZ derivative were grown at 42°C for 30 min and then induced with 200 μM IPTG for 60 min. Parallel samples were fixed and stained with anti-GFP antibodies for FtsA-GFP and stained with anti-FtsZ antibodies for FtsZ, followed by IFM. (A to E) FtsA-GFP; (A′ to E′) FtsZ. (A and A′) WM1234 (JFL101 containing pWM633 [ftsA-GFP] and pWM176); (B and B′) WM1235 (pMK4 [wild-type ftsZ]); (C and C′) WM1242 (pWM738 [ZD373A]); (D and D′) WM1244 (pWM739 [ZP375A]); (E and E′) WM1236 (pWM737 [ZΔC1]).