TABLE 1.
Strains and plasmids used in this study
| Bacterial strain or plasmid | Relevant characteristics | Source and/or reference |
|---|---|---|
| JM105 | F′ traD36 lacIq Δ(lacZ)M15 proA+B+/thi rpsL endA sbcB15 hsdR4 Δ(lac proAB) | Laboratory collection |
| JFL101 | ftsZ84 recA lacY | 18 |
| WM746 | WX7 (ftsZ frameshift mutant) containing pCX41 (temperature-sensitive plasmid carrying ftsZ) | 35 |
| CAG12131 | leu::Tn5 | Laboratory collection |
| WM1099 | Tets version of WM746; WM746 × P1 (CAG12131; leu::Tn5) | Laboratory collection |
| WM1221 | WM1099 containing pWM1206 + pWM176; Tetr Ampr | This work |
| WM1222 | WM1099 containing pWM1206 + pMK4; Tetr Ampr | This work |
| WM1223 | WM1099 containing pWM1206 + pWM737; Tetr Ampr | This work |
| WM1224 | WM1099 containing pWM1206 + pWM930; Tetr Ampr | This work |
| WM1225 | WM1099 containing pWM1206 + pWM931; Tetr Ampr | This work |
| WM1226 | WM1099 containing pWM1206 + pWM932; Tetr Ampr | This work |
| WM1227 | WM1099 containing pWM1206 + pWM1201; Tetr Ampr | This work |
| WM1228 | WM1099 containing pWM1206 + pWM1202; Tetr Ampr | This work |
| WM1229 | WM1099 containing pWM1206 + pWM738; Tetr Ampr | This work |
| WM1230 | WM1099 containing pWM1206 + pWM1203; Tetr Ampr | This work |
| WM1231 | WM1099 containing pWM1206 + pWM739; Tetr Ampr | This work |
| WM1232 | WM1099 containing pWM1206 + pWM1204; Tetr Ampr | This work |
| WM1233 | WM1099 containing pWM1206 + pWM1205; Tetr Ampr | This work |
| WM1234 | JFL101 containing pWM633 + pWM176; Camr Tetr | This work |
| WM1235 | JFL101 containing pWM633 + pMK4; Camr Tetr | This work |
| WM1236 | JFL101 containing pWM633 + pWM737; Camr Tetr | This work |
| WM1237 | JFL101 containing pWM633 + pWM930; Camr Tetr | This work |
| WM1238 | JFL101 containing pWM633 + pWM931; Camr Tetr | This work |
| WM1239 | JFL101 containing pWM633 + pWM932; Camr Tetr | This work |
| WM1240 | JFL101 containing pWM633 + pWM1201; Camr Tetr | This work |
| WM1241 | JFL101 containing pWM633 + pWM1202; Camr Tetr | This work |
| WM1242 | JFL101 containing pWM633 + pWM738; Camr Tetr | This work |
| WM1243 | JFL101 containing pWM633 + pWM1203; Camr Tetr | This work |
| WM1244 | JFL101 containing pWM633 + pWM739; Camr Tetr | This work |
| WM1245 | JFL101 containing pWM633 + pWM1204; Camr Tetr | This work |
| WM1246 | JFL101 containing pWM633 + pWM1205; Camr Tetr | This work |
| Plasmid | ||
| pBAD30 | araBAD promoter vector; p15A origin; Ampr | Laboratory collection |
| pBCIISK | colE1 cloning vector; Camr | Stratagene |
| pBC (SK+) | colE1 cloning vector; Camr | Stratagene |
| pZAQ | pBR322 containing ftsQAZ insert between PstI and ClaI; Tetr | 36 |
| pZG | Camr; ftsZ-GFP cloned between SacI and PstI sites in pBC SK(+) | 19 |
| pGBC | Camr; GFP cloned between BamHI and XbaI sites in pBC SK(+) | 21 |
| pWM707 | Camr; ZΔC1 inserted between SacI and XbaI sites in pGBC | This work |
| pWM630 | Camr; pGBC-ZΔC2 | 19 |
| pWM766 | Camr; pGBC-ZΔC3 | This work |
| pWM767 | Camr; pGBC-ZΔC4 | This work |
| pWM1206 | Ampr; zipA-GFP-lacIq fragment cloned between SacI and SalI sites of pBAD30 | This work |
| pWM1207 | Ampr; pBluescript containing ftsA and lacIq | This work |
| pWM633 | Camr; pGBC containing ftsA-GFP and lacIq | This work |
| pWM176 | Tetr; IncP cloning vector containing the tac promoter | 24 |
| pMK4 | Tetr; E. coli wild-type ftsZ inserted at XhoI and SacI sites of pWM176 | 24 |
| pWM737 | Tetr; pWM176-ZΔC1 | This work |
| pWM930 | Tetr; pWM176-ZΔC1.1 | This work |
| pWM931 | Tetr; pWM176-ZΔC1.2 | This work |
| pWM932 | Tetr; pWM176-ZΔC1.3 | This work |
| pWM1201 | Tetr; pWM176-ZΔC2 | This work |
| pWM1202 | Tetr; pWM176-ZL372A | This work |
| pWM738 | Tetr; pWM176-ZD373A | This work |
| pWM1203 | Tetr; pWM176-ZI374A | This work |
| pWM739 | Tetr; pWM176-ZP375A | This work |
| pWM1204 | Tetr; pWM176-ZF377A | This work |
| pWM1205 | Tetr; pWM176-ZL378A | This work |
| pWM1208 | pLEXA-ftsA | This work |
| pXZE209a | pACT2.2-virE (from A. tumefaciens) | P. Christie |
| pWM1209 | pACT2.2-ftsZ | This work |
| pWM1210 | pACT2.2-ZΔC1 | This work |
| pWM1211 | pACT2.2-ZΔC1.1 | This work |
| pWM1212 | pACT2.2-ZΔC1.2 | This work |
| pWM1213 | pACT2.2-ZΔC1.3 | This work |
| pWM1214 | pACT2.2-ZΔC2 | This work |
| pWM1215 | pACT2.2-ZL372A | This work |
| pWM1216 | pACT2.2-ZD373A | This work |
| pWM1217 | pACT2.2-ZI374A | This work |
| pWM1218 | pACT2.2-ZP375A | This work |
| pWM1219 | pACT2.2-ZF377A | This work |
| pWM1220 | pACT2.2-ZL378A | This work |