TABLE 5.
Summary of interactions between FtsA and FtsZ derivatives
| Derivativea | Interaction with FtsA
|
Interaction with ZipAd | |
|---|---|---|---|
| E. coli in vivo assayb | Yeast two-hybridc | ||
| FtsZ | + | ++ | + |
| ZL372A | − | − | + |
| ZD373A | + | + | + |
| ZI374A* | − | − | − |
| ZP375A | − | − | + |
| ZF377A* | − | − | − |
| ZL378A | + | NT | + |
| ZΔC1 | − | − | − |
| ZΔC1.1 | − | − | − |
| ZΔC1.2 | − | − | − |
| ZΔC1.3 | − | − | − |
| ZΔC2 | − | − | − |
* indicates that the FtsZ derivative is unstable.
The FtsA-FtsZ interaction shown was tested in JFL101 (ftsZ84). +, strong fluorescent spirals; −, no strong fluorescent spirals.
See legend to Table 4 for explanation of the data.
The results of ZipA-FtsZ interaction shown here were examined in both JFL101 (ftsZ84) and WM1099 (ftsZ depletion) strains. NT, not tested.