TABLE 5.
Summary of interactions between FtsA and FtsZ derivatives
Derivativea | Interaction with FtsA
|
Interaction with ZipAd | |
---|---|---|---|
E. coli in vivo assayb | Yeast two-hybridc | ||
FtsZ | + | ++ | + |
ZL372A | − | − | + |
ZD373A | + | + | + |
ZI374A* | − | − | − |
ZP375A | − | − | + |
ZF377A* | − | − | − |
ZL378A | + | NT | + |
ZΔC1 | − | − | − |
ZΔC1.1 | − | − | − |
ZΔC1.2 | − | − | − |
ZΔC1.3 | − | − | − |
ZΔC2 | − | − | − |
* indicates that the FtsZ derivative is unstable.
The FtsA-FtsZ interaction shown was tested in JFL101 (ftsZ84). +, strong fluorescent spirals; −, no strong fluorescent spirals.
See legend to Table 4 for explanation of the data.
The results of ZipA-FtsZ interaction shown here were examined in both JFL101 (ftsZ84) and WM1099 (ftsZ depletion) strains. NT, not tested.