FIG. 5.
HasA and LipA secretion by hybrid Has exporters. The plasmids pACYC/HasDPF and pACYC/HasDSM were used as the plasmids encoding the ABC proteins HasDPF and HasDSM8000, respectively. MFP and OMP were provided by pMW/HasEFPF (hasEFPF) and pMW/HasESM. These plasmids were introduced into the E. coli cells harboring the LipAPF plasmid pUC/PFLipA13 (A), the HasAPF plasmid pUC/HasAPF (B), and the HasASM plasmid pUC/HasASM (C). The cells were cultured and tested for protein secretion as described in the legend to Fig. 3. The gels were analyzed by immunoblotting with antisera against LipASM, HasAPF, and HasASM. Secretory proteins are indicated on the left and optical density (O.D.) equivalents are shown below. Lanes 1, pACYC184 plus pMW/HasESM; lanes 2, pACYC184 plus pMW/HasEFPF; lanes 3, pACYC/HasDSM plus pMW/HasESM; lanes 4, pACYC/HasDSM plus pMW/HasEFPF; lanes 5, pACYC/HasDPF plus pMW/HasESM; lanes 6, pACYC/HasDPF plus pMW/HasEFPF.