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. 2022 May 30;34(9):3319–3338. doi: 10.1093/plcell/koac161

Figure 1.

Figure 1

RLI1 generates two transcripts and their corresponding protein isoforms. A, Gene structure of RLI1 and the primers used to identify two different RLI1 splicing variants. Boxes indicate exons, solid lines indicate introns, dotted lines indicate splicing sites of introns, and the box with dots indicates the intron for RLI1b. “ATG” indicates the start codon and “TAG” indicates the stop codon. F is the forward primer. Ra is the reverse primer for RLI1a amplification. Rb is the reverse primer for RLI1b amplification. bp, base pairs. B, Protein structures of RLI1a and RLI1b. MYB indicates the DNA binding domain of RLI1a and RLI1b. CC indicates the CC domain. C, RT-PCR analysis of the different transcript isoforms of RLI1. D, Immunoblot analysis of the protein isoforms of RLI1a and RLI1b. The nuclear proteins of the WT (NIP) and rli1-1 were isolated for immunoblot assays with anti-RLI1 antibody. Coomassie brilliant blue (CBB) staining indicates that similar amounts of proteins were loaded. * indicates nonspecific bands.