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. 2022 Jun 7;34(9):3301–3318. doi: 10.1093/plcell/koac169

Figure 6.

Figure 6

DHT1 regulates SL signaling. A, Comparison of epi-5DS levels in WT and dht1 root exudates. g FW is indicated as per gram fresh weight. B, Response of tiller buds in WT, dht1 and d14 to 1 μM GR24 treatment. Red arrows indicate the elongated tiller buds. Scale bars, 2 cm. C, Expression levels of DHT1 in five recessive d mutants (d27, d17, d10, d3, and d14) with Kitaake background and the expression level of DHT1 in the dominant d53 mutant with Norin 8 background. D, Immunoblot showing lower D14 protein levels in the tiller buds of dht1 compared with that of WT plants. E, Immunoblot showing higher D53 protein levels in the tiller buds of dht1 and DHT1 knockout plants (CR1 and CR2) compared with these of WT plants. F, Immunoblot showing D53 protein levels in WT, CR1 and d14 mutant plants after 3 h treatment with 5 µM GR24. G, Tiller number of WT, dht1 and dht1D14-3xFlag overexpression transgenic plants at the mature stage. H, Tiller number of WT, dht1 and dht1D53-RNAi transgenic plants at the mature stage. I, Immunoblot showing D14 and D53 protein levels in the tiller buds of WT, dht1, and dht1D14-3xFlag overexpression transgenic plants. J, Immunoblot showing D53 levels in WT, dht1, and dht1D53-RNAi transgenic plants. K, Tiller number of WT, dht1 and dht1D53-CRISPR transgenic plants at the mature stage. L, Phenotypes of WT, dht1, dht1D14-3xFlag, dht1D53-RNAi, and dht1D53-CRISPR transgenic plants at the mature stage. Scale bars, 5 cm. Data are means ± sem (n = 3) and P-values are from two-sided Student’s t test (A and C). Data are means ± sem (n = 7) and P-values are from one-way ANOVA test with Tukey's correction (G, H, and K). Anti-HSP82 was used as a loading control in (D, E, F, I, and J).