Figure 1.

Complexity increases from the genome to the interactome in human cells

(A) Interactome complexity is generated at the genome, transcriptome, and proteome levels, with alternative splicing and post-translational modifications (PTMs) among other features that contribute to protein-protein interaction (PPI) diversity.

(B) Y2H and AP-MS have been used to define PPI networks of human proteins. Tens of thousands of interactions have been reported, but a large portion of the human interactome has remained uncharacterized, known as the dark interactome, which is depicted in dark gray. The table provides the biological and cellular context and examples of proteins within the dark interactome.