Figure 3.

Proposed strategy for uncovering cell-type-specific PPIs

(A) Prior to an interactome study, the expression pattern of the gene of interest (GOI) is investigated using data repositories such as the Human Protein Atlas (HPA), Geno-type-Tissue Expression (GTEx), or Gene Expression Atlas (GEA). An appropriate mammalian expression viral vector is used to express the GOI in frame with a BirA enzyme (e.g., TurboID) for PL-MS.

(B) Transfection or transduction is carried out using an immortalized tissue-specific cell type, tissue-specific or primary stem cell line such as embryonic stem cells (ESCs) and mesenchymal stromal cells (MSCs), or their derivative cell types.

(C) Once MS-based interactome studies are conducted, cell-type-specific maps can be generated.