Figure 15.

Characterization of stromal cell alterations in Six2-Cre^+/--PKAcαR^fl/wt;R-tdTomato^fl/wtcorpus.A–F, Corpus sections from 8M-old Six2-Cre^+/-;PKA^wt/wt; R-tdTomato^fl/wt (control) and Six2-Cre^+/--PKAcαR^fl/wt;R-tdTomato^fl/wt (mutant) mice co-stained with RFP (green) (staining tdTomato) and ACTA2 antisera (red). In control (A–C), RFP (arrows) and ACTA2 (arrowheads) double-positive stromal cells present underneath, in between gastric glands and in the muscularis mucosae are indicated. In mutant (D–F), RFP/ACTA2 double-positive cell population is markedly expanded (arrowheads) and is present underneath and in between invading and cystic glands. G–L, Corpus sections from 8M-old Six2-Cre^+/-;PKA^wt/wt; R-tdTomato^fl/wt (control) and Six2-Cre^+/--PKAcαR^fl/wt;R-tdTomato^fl/wt (mutant) mice co-stained with RFP (green) (staining tdTomato) and VIM antisera (red). In control (G–I), RFP (arrows) and VIM (arrowheads) double-positive stromal cells present underneath, in between gastric glands and in the muscularis mucosae are indicated. In mutant (J–L), RFP/VIM double-positive cell population is markedly expanded (arrowheads) and is present underneath and in between invading and cystic glands. M–R, Corpus sections from 8M-old Six2-Cre^+/-;PKA^wt/wt; R-tdTomato^fl/wt (control) and Six2-Cre^+/--PKAcαR^fl/wt; R-tdTomato^fl/wt (mutant) mice co-stained with RFP (green) (staining tdTomato) and PDGFRA antisera (red). In control (M–O), RFP⁺/PDGFRA^- (green arrows), RFP^-/PDGFRA⁺ (red arrowheads), and RFP⁺/PDGFRA⁺ (white arrows) cells present in between gastric glands are indicated. In mutant (P–R), RFP^-/PDGFRA⁺ (red arrowheads) expanded cell population localized around the glands is indicated. RFP⁺/PDGFRA^- (green arrows), was expanded present in between cystic glands is indicated. Relatively fewer RFP⁺/PDGFRA⁺ (concave arrowheads) cells are indicated. Scale bars = 50 μm. Blue (DAPI).