Figure 3.

Six2-Cre driven tdTomato expression is restricted to the mesenchymal compartment of all 3 regions of the stomach.A–F, Images of 3 regions of stomach from neonatal control and CA-PKA mice co-stained with antisera against RFP (green) and alpha smooth muscle actin (ACTA2) (red); nuclei are stained with DAPI. In the forestomach (A), corpus (B), and antrum (C) regions of control Six2-Cre^-/-R-tdTomato^fl/wt stomach, no RFP signal was observed; ACTA2-stained mesenchymal cells of all 3 stomach regions are indicated (arrows). In Six2-Cre^+/-R-tdTomato^fl/wt stomach (D–F), RFP signal observed in the lamina propria submucosa, muscularis mucosae, and muscularis externa of stomach (arrowheads) overlapped almost completely ACTA2-stained cells of all 3 stomach regions. G–L, Images of 3 regions of stomach from neonatal control and CA-PKA mice co-stained with antisera against RFP (green) and ECAD (red); nuclei are stained with DAPI. In the forestomach (G), corpus (H), and antrum (I) regions of control Six2-Cre^-/-R-tdTomato^fl/wt stomach, no RFP signal was observed; ECAD-stained epithelial cells of all 3 stomach regions are indicated (arrows). In Six2-Cre^+/-R-tdTomato^fl/wt stomach (J–L) RFP signal in the lamina propria (arrowheads) and developing muscularis externa (arrowheads) is indicated; no RFP signal (green) was observed in ECAD-stained epithelial cells of all 3 stomach regions (arrows). Scale bars = 50 μm