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. 2022 Jun 9;14(3):643–668.e1. doi: 10.1016/j.jcmgh.2022.06.001

Figure 5.

Figure 5

Cellular alterations in CA-PKA mutant stomach.A–D, Stomach from 6-week- and 12-month-old control and CA-PKA mice co-stained with lectins UEA1 (red) and GSII (green). In control (A, C), UEA1 in pit cells (arrows) and GSII signal in mucous neck cells (arrowheads) are shown. In mutant (B, D) UEA1/GSII double-positive cells (arrowheads) and cystic glands (dotted lines) containing both UEA1+ and GSII+ cells (arrows) are shown. E–F, Stomach from 12-month-old control (E) and CA-PKA (F) mice co-stained with MUC5B (red) and GSII-lectin (green). In control, MUC5B (arrows) and GSII (arrowheads) stained distinct cells. In the CA-PKA corpus, increased MUC5B/GSII double-positive cells (arrowheads) and remaining MUC5B+/GSII- cells (arrows) are shown. G, Representative immunoblots and quantification graphs (∗P < .05) showing gastrokine1 (n = 3), TFF2 (n = 4), and MUC5B (n = 4) relative levels in corpus extracts of control and CA-PKA mice. H–K, Stomach from control and CA-PKA mutant co-stained with MIST1 (red) and DBA-lectin (green). In controls (H, J), MIST1+ chief cells (arrowheads) and DBA+ parietal cells (arrows) are shown. In 6-week-old mutant (I) DBA+ (arrows) and MIST1+ cells (arrowheads) were fewer. In 12-month-old mutant (K), glands containing severely reduced (arrows) or no DBA+ cells (arrowheads) are indicated. L–M, Stomach sections from control and mutant co-stained with parietal cell marker, ATP4A (red, arrows) and GSII-lectin (green, arrowheads). In control (L) ATP4A+ (arrows) and GSII+ (arrowheads) cells are indicated. Mutant glands (M) containing severely reduced (arrowheads) or no ATP4A+ cells (arrows) are indicated. N, Representative immunoblots and quantification graphs (∗P < .05) showing ATP4A (n = 4) and MIST1 (n = 3) relative levels in mutant corpus vs control. Scale bars = 50μm. Blue (DAPI).