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. 2022 Jun 16;34(9):3383–3399. doi: 10.1093/plcell/koac180

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© The Author(s) 2022. Published by Oxford University Press on behalf of American Society of Plant Biologists.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Accumulation of ROS upon repression of Orf2971. A, Scatter plot of ROS-induced DCF-DA fluorescence from samples with 400,000 cells of A31 and Y28 in the light and 100,000 cells in the dark in the absence or presence of vitamins showing coincidence of elevated ROS. FSC-Height (Y-axis) indicates cell size and cell surface area, FL1-Log-Height (X-axis) indicates ROS fluorescence intensity. B, Levels of expression of ROS-related marker genes in the light or dark upon Orf2971 repression determined by qPCR. Data are represented as mean ± sd. C, Analysis of proteins from Y28 grown in the light or dark upon Orf2971 repression. Proteins were extracted at different time points and immunoblotted with the antisera as indicated on the left.