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. 2022 Jun 16;34(9):3383–3399. doi: 10.1093/plcell/koac180

Figure 5.

Figure 5

Orf2971 is associated with the TIC complex and protein folding components in the chloroplast. A, Membrane fractions (left) and soluble fractions (right) of the gradients were collected from WT and analyzed by SDS–PAGE and immunoblotting. B, Fractions of the gradients of the membrane (left) and soluble phase (right) were collected from WT and analyzed by 2D BN/SDS–PAGE. C, Immunoprecipitation of FLAG-tagged Orf2971 and associated proteins. Insoluble (left) and soluble (right) extracts of FLAG-tagged Orf2971 and WT strains were co-immunoprecipitated with anti-Flag antibody. The immunoprecipitated proteins were separated by SDS–PAGE and detected by immunoblotting with anti-Orf2971 antibody and the indicated antibodies. YC-FLAG, FLAG-tagged Orf2971 strain. D, Classification of proteins interacting with Orf2971 based on co-immunoprecipitation and mass-spectrometry reveals two categories involved in protein translocation across the chloroplast envelope and in the folding of chloroplast proteins. E, Schematic illustration of Orf2971 interactions with chloroplast proteins.