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. 2022 Jun 16;34(9):3383–3399. doi: 10.1093/plcell/koac180

Figure 6.

Figure 6

Detection of localization of nucleus-encoded RBCS2 and chloroplast precursor proteins upon Orf2971 depletion. A, Confocal microscopy of Y28-RBCS2-Venus in the presence (Orf2971 OFF) or absence (Orf2971 ON) of vitamins for different times. B, Schematic representation of the putative chloroplast protein transit peptides (cTP). The scale at the top indicates the number of amino acids. C, Chart summarizing the classifications based on the proteins shown in (A). D, Immunoblot analysis of the HSP22E/F and HPS70B precursor with or without MG132 treatment during Orf2971 depletion. The asterisks indicate putative accumulated preproteins.