TABLE 1.
Strains, plasmids, and phages used in this study
| Strain, plasmid, or phage | Relevant genotype | Source or reference |
|---|---|---|
| Strains | ||
| ECL3999 | MC4100 but adhE::kan | 18 |
| ECL4010 | Same as MC4100 but λADHpr291 | This study |
| ECL4011 | Same as MC4100 but λADHop291 | This study |
| ECL4012 | Same as MC4100 but λADHpr656 | This study |
| ECL4013 | Same as MC4100 but λADHop656 | This study |
| ECL4014 | Same as MC4100 but λADHop2656 | This study |
| ECL4053 | Same as MC4100 but λADHop3656 | This study |
| ECL4015 | Same as ECL4011 but rnc::Tn10 | P1(RS6521) × ECL4011 |
| ECL4016 | Same as ECL4012 but rnc::Tn10 | P1(RS6521) × ECL4012 |
| ECL4017 | Same as ECL4013 but rnc::Tn10 | P1(RS6521) × ECL4013 |
| ECL4018 | Same as ECL4010 but rnc::Tn10 | P1(RS6521) × ECL4010 |
| ECL4020 | Same as ECL4011 but fnr-271::Tn10 | P1(VJS1741) × ECL4011 |
| ECL4022 | Same as ECL4013 but fnr-271::Tn10 | P1(VJS1741) × ECL4013 |
| ECL4024 | Same as ECL4011 but cra::Tn10 | P1(LJ2805) × ECL4011 |
| ECL4027 | Same as ECL4011 but fis::kan | P1(RJ708) × ECL4011 |
| ECL4028 | Same as ECL4013 but fis::kan | P1(RJ708) × ECL4013 |
| ECL4031 | Same as ECL4011 but appY::aphA | P1(TC3572) × ECL4011 |
| ECL4032 | Same as ECL4013 but appY::aphA | P1(TC3572) × ECL4013 |
| ECL4033 | Same as ECL4011 but narL::Tn10 | P1(RK5267) × ECL4011 |
| ECL4034 | Same as ECL4013 but narL::Tn10 | P1(RK5267) × ECL4013 |
| ECL4055 | Same as ECL4011 but rpoS::kan | P1(ECL1226) × ECL4011 |
| ECL4056 | Same as ECL4011 but rpoS::kan fnr-271::Tn10 | P1(ECL1226) × ECL4020 |
| ECL4054 | Same as MC4100 but λADHop656TATA | This study |
| LJ2805 | cra::Tn10 | 6 |
| MC4100 | F′ ΔlacU169 rpsL150 | 5 |
| RJ708 | fis::kan | Reid Johnson |
| RK5267 | narL::Tn10 | Valley Stewart |
| RS6521 | rnc::Tn10 | R. W. Simons |
| TC3572 | appY::aphA | 3 |
| VJS1741 | fnr-271::Tn10 | Valley Stewart |
| ECL1226 | rpoS::kan | Laboratory collection |
| Plasmids | ||
| pADH8 | ApradhE+ | 5 |
| pADHop2656 | pRS415/ADHop2656a | This study |
| pADHop291 | pRS415/ADHop291a | This study |
| pADHop656 | pRS415/ADHop656a | This study |
| pADHpr291 | pRS414/ADHpr291a | This study |
| pADHpr656 | pRS414/ADHpr656a | This study |
| pRS414 | AprlacZYA+ (protein fusion) | 25 |
| pRS415 | AprlacZYA+ (operon fusion) | 25 |
| pJAMDH1 | pBR322/adhE+ up to −656 | This study |
| pJMADH2 | pBR322/adhE+ up to −291 | This study |
| pJMADH3 | pBR322/adhE+ up to −656 but Pribnow TACAAT box (from positions −304 to −298) changed to CGGGCC | This study |
| Phages | ||
| λADHop291 | (adhE-lacZ)b | This study |
| λADHop656 | (adhE-lacZ)b | This study |
| λADHpr291 | (adhE-lacZ)b | This study |
| λADHpr656 | (adhE-lacZ)b | This study |
| λADHop2656 | (adhE-lacZ)b | This study |
| λADHop3656 | (adhE-lacZ)b | This study |