TABLE 3.
Aerobic and anaerobic β-galactosidase activity of Φ(adhE-lacZ)291 in the presence of 40 mM nitrate and a narL mutationa
| Strain | Relevant genotype | β-Galactosidase activity (Miller units)
|
|||
|---|---|---|---|---|---|
| +O2 | −O2 | +NO3
|
|||
| +O2 | −O2 | ||||
| ECL4011 | Φ(adhE-lacZ)291 | 312 ± 15 | 2,815 ± 49 | 129 ± 10 | 151 ± 29 |
| ECL4033 | Φ(adhE-lacZ)291 narL::Tn10 | 326 ± 23 | 3,153 ± 18 | 188 ± 25 | 2,993 ± 23 |
The cells were grown in LB-glucose medium and assayed in the exponential phase of growth (OD600, 0.4).