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Reconstitution of active rDld after extraction of cofactor. rDld was electrophoresed under denaturing conditions, allowed to renature with or without flavin cofactor, and then assayed as described in Materials and Methods. Lanes: 1, renaturation with FMN-riboflavin; 2, renaturation with F420; 3, no cofactor added; 4, renaturation with FAD; 5, Benchmark protein standards.
