TABLE 1.

R_f values of flavins by ascending paper chromatography^a

Solvent	Rf value
	Flavin standard			Dld cofactorb
	FMN	Riboflavin	FAD
Ac	0.50	0.28	0.35	0.34
Bd	0.23	0.39	0.09	0.10

^aSamples were separated on Whatman no. 1 paper as described in the text. 

^brDld cofactor was released by boiling. Apoprotein was removed by centrifugation. 

^cSolvent system A; 5% disodium hydrogen phosphate in H₂O. 

^dSolvent system B; n-butanol–acetic acid–H₂O (4:1:5).