TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic | Source or reference |
|---|---|---|
| Strains | ||
| Lou15 | V. cholerae El Tor | 42 |
| O395 | V. cholerae classical | S. Calderwood |
| EWV103 | V. cholerae Lou15 viuP::cam | This study |
| SSV121 | V. cholerae Lou15 viuG::cam | This study |
| MBG14 | V. cholerae O395 viuA::TnphoA | 16 |
| DH5α | E. coli cloning strain, Ent+ | 18 |
| SM10λpir | E. coli host for pHM5 | 29 |
| AB1515.414 | E. coli AB1515 fepB::Tn5 | C. Earhart |
| AB1515.718 | E. coli AB1515 fepD::Tn5 | C. Earhart (10) |
| AB1515.764 | E. coli AB1515 fepG::Tn5 | C. Earhart (10) |
| AB1515.199 | E. coli AB1515 fepC::Tn5 | C. Earhart (10) |
| Plasmids | ||
| pBluescript SK− | Cloning vector | Stratagene |
| pSU18 | Cloning vector | 28 |
| pWSK29 | Cloning vector | 50 |
| pWKS30 | Cloning vector | 50 |
| pMA9 | cam resistance gene | 24 |
| pMTLcam | cam resistance gene in the BamHI site of pMTL24 | 54 |
| pWSc1 | pWKS30 carrying sacB | 31 |
| pHM5 | pGP704 carrying sacB | 37 |
| pCP111 | pACYC184 with an 11-kbp EcoRI fragment carrying fepBDGC in the EcoRI site | 33 |
| pJSV90 | pACYC184 with a 9-kbp EcoRI fragment carrying viuPDGC and vibD and vibH in the EcoRI site | 53 |
| pVIB109 | 5.5-kbp PstI-SalI fragment of pJSV90 subcloned into the PstI-SalI sites of pSU18 | This study |
| pVIB119 | 2.9-kbp BamHI fragment containing vibH and viuP subcloned into the BamHI site of pBluescript SK− | This study |
| pVIB121 | HindIII fragment of pVIB109 cloned into the HindIII site of pBluescript SK− | This study |
| pVIB147 | BglII fragment from pJSV90 subcloned in the BamHI site of pWKS30 | This study |
| pVIB148 | pVIB119 with a cam cassette inserted in the MscI site in viuP | This study |
| pVIB154 | pVIB147 with a cam cassette inserted in the MscI site in viuP | This study |
| pVIB159 | pVIB147 from which sequences to the right of the EcoRV site were deleted | This study |
| pViuAB | PCR fragment containing viuA and viuB subcloned in the SmaI site of pWSK29 | This study |
| pUNK143 | pHM5 containing viuG::cam | This study |