TABLE 3.
ViuPDGC, but not FepBDGC, transports vibriobactin in E. coli
| Indicator strain (plasmid-encoded genes) | Zone of stimulation (mm) witha:
|
||
|---|---|---|---|
| Lou15 | DH5α | FeSO4 | |
| fepBb (viuAB) | 14c | 0 | 21 |
| fepB (viuAB and viuPDGC) | 40 | 26 | 38 |
| fepB (viuAB and fepBDGC) | 0 | 27 | 35 |
| fepD (viuAB) | 18c | 0 | 28 |
| fepD (viuAB and viuPDGC) | 36 | 25 | 29 |
| fepD (viuAB and fepBDGC) | 0 | 38 | 40 |
a
Cultures of the indicator strains were seeded into L agar containing 300 μg of EDDA per ml. V. cholerae Lou15, E. coli DH5α, or 10 mM FeSO4 was spotted onto the medium. The zone of growth of the indicator strains was measured 18 h after inoculation.
b
The fepB strain used was AB1515.414 and the fepD strain was AB1515.718. The plasmid carrying the viuAB was pViuAB; the plasmid carrying viuPDGC was pJSV90; the plasmid carrying fepBDGC was pCP111.
c
A very sparse zone of growth was observed around vibriobactin in indicator strains that do not transport enterobactin. The density of colonies within these zones was much lower than those observed in other zones (see text).