Table 1.

Common human pathogen targets and primer sequences.

Pathogens	Genes	Enzymes	Primer sequences	PCR product	References
Enterobacteriaceae (K. pneumoniae／E. coli／Salmonella)	blaCMY	AmpC β-lactamase	5′-CTGCTGCTGACAGCCTCTTT-3′	1109 bp	[25]
			5′-TTTTCAAGAATGCGCCAGGC-3′
	blaDHA	AmpC β-lactamase	5′-CGTCTGACCATAATCCACCTGT-3′	1300 bp	[26]
			5′-CCAGTGCACTCAAAATAGCCT-3′
	blaCTX-M-3	ESBL	5′-CCAGAATAAGGAATCCCAT-3′	911 bp	[27]
			5′-CCCATTCCGTTTCCGCTA-3′
	blaCTX-M-14	ESBL	5′-AACACGGATTGACCGTCTTG-3′	906 bp	[28]
			5′-TTACAGCCCTTCGGCGAT-3′
	blaSHV	ESBL	5′-GGGTTATTCTTATTTGTCGC-3′	928 bp	[29]
			5′-TTAGCGTTGCCAGTGCTC-3′
	mcr-1	phosphoethanolamine transferases	5ʹ-CGGTCAGTCCGTTTGTTC-3ʹ	308 bp	[30]
			5ʹ-CTTGGTCGGTCTGTAGGG-3ʹ
	blaNDM	Class B metallo-β-lactamase	5′-ACGGTTTGGCGATCTGGTTT-3′	663 bp	GenBank: FN396876.1
			5′-ATGCGGGCCGTATGAGTGATT-3′
	blaKPC	Class A metallo-β-lactamase	5′-CGCCGTCTAGTTCTGCTGTCTTGT-3′	879 bp	GenBank: AF297554.1
			5′-TTCAGAGCCTTACTGCCCGTTGAC-3′
P. aeruginosa	blaVIM	Class B metallo-β-lactamase	5′-GATGGTGTTTGGTYGCATA-3′	390 bp	GenBank: Y18050.2
			5′-CGAATGCGCAGCACCRG-3′
	algD	GDP mannose dehydrogenase gene	5′-TTCCCTCGCAGAGAAAACATC-3′	520 bp	[31]
			5′-CCTGGTTGATCAGGTCGATCT-3′
	exoT	Exotoxin T	5′-AATCGCCGTCCAACTGCATGCG-3′	152 bp	[32]
			5′-TGTTCGCCGAGGTACTGCTC-3′